Methods used in protein characterization

For characterizing protein solutions, the following methods are available in our biological laboratory:

• Viscosity determination (low-viscosity rolling-ball rheometer Lovis-2000-M-ME),
• Infrared spectroscopy, suitable for the analysis of secondary structures (FTIR Tensor 27 for secondary structure analysis)
• DLS to determine diffusion and aggregation (Dynamic Light Scattering Zetasizer Nano ZS (Malvern))

• UV-spectroscopy to measure concentrations (UV-Vis NanoDrop 2000c (Thermo Scientific))